Table 3.
List of antibodies used in Western blot analyses.
| Host/clonality | Clone | Catalog numer/RRID | Source | Dilution | |
|---|---|---|---|---|---|
| Primary | |||||
| HSPA1 | Mo/M | C92F3A-5 | ADI-SPA-810-F/AB_311860 | Enzo, Life Sciences, Famingdale, NY | 1:5000 |
| HSPA2 | Ra/M | EPR4596 | Ab108416/AB_10862351 | Abcam, Cambridge, UK | 1:5000 |
| HSPA5 | Mo/M | A-10 | Sc-376768/nd | Santa Cruz Biotechnology, Inc., Dallas, USA | 1:1000 |
| HSPA8 | Mo/M | B-6 | Sc-7298/AB_627761 | Santa Cruz Biotechnology, Inc., Dallas, USA | 1:7500 |
| HSPC | Mo/M | AC88 | ADI-SPA-830-F/AB_11181197 | Enzo, Life Sciences, Famingdale, NY | 1:1000 |
| BAG1 | Mo/M | 19 | 611868/AB_399348 | BD Biosciences, San Diego, CA | 1:1000 |
| BAG3 | Mo/M | 19 | Ssc-136467/AB_10647772 | Santa Cruz Biotechnology, Inc., Dallas, USA | 1:1000 |
| cleaved caspase-3 | Ra/M | 5A1E | 9664/AB_2070042 | Cell Signaling Technology, | 1:2000 |
| cleaved PARP | Ra/M | D64E10 | 5625/AB_10699459 | Cell Signaling Technology, | 1:2000 |
| β-actin (HRP) | Mo/M | AC15 | A3854/AB_262011 | Merck KGaA, Darmstadt, Germany | 1:20000 |
| Secondary | |||||
| Anti-Mo IgG (HRP) | Go | AP124P/AB_90456 | Millipore, Billerica, MA | 1:5000 | |
| Anti-Ra IgG (HRP) | Go | AP132P/AB_90264 | Millipore, Billerica, MA | 1:2000 | |
Abbreviations: RRID, Research Resource Identifier; WB, Western Blot; M, monoclonal; Go, goat; Mo, mouse; nd, no data; Ra, Rabbit; HRP, horseradish peroxidase.