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. 2019 Oct 7;10(10):758. doi: 10.1038/s41419-019-1994-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic diagram of the animal study. NASH was induced with a MCD diet for 8 weeks. Ad.CON, control adenovirus + MCD diet; Ad.PGRN, PGRN-expressing adenovirus + MCD diet; TA, tibialis anterior. b Serum PGRN levels. c Final body, liver, and spleen weights of Ad.CON and Ad.PGRN mice. d Serum ALT and AST levels. e Liver sections were stained with H&E and Sirius Red. Scale bar: 200 μm. f Quantification of steatosis, inflammatory foci, and Sirius Red staining. Graphs show the mean ± SEM. *p < 0.05 and **p < 0.01 versus the corresponding control, as shown