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. 2019 Oct 7;10(10):758. doi: 10.1038/s41419-019-1994-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Raw 264.7 cells and primary macrophages were pre-treated with PGRN for 30 min. They were then treated with palmitate for an additional hour, and expression of inflammatory genes was analyzed by real-time qPCR. c Raw 264.7 cells were treated with conditioned media from HepG2 or Huh7 cells treated with palmitate for 6 h with or without 30 min of PGRN pre-treatment. Raw 264.7 cells were exposed to the conditioned media for 1 h and then subjected to real-time qPCR analysis. Graphs show the mean ± SEM. *p < 0.05 and **p < 0.01 versus the corresponding control, as shown