Figure 1.

Effect of purinergic agonists and KCl‐induced depolarization on PKD1 activation. (A) Representative immunoblots of protein extracts from mouse islets stimulated for 20 min with the P2Y₁ agonists, MRS2365 (100 nmol/L) or ATP (10 μmol/L) at 1 mmol/L glucose and analyzed by Western blot for phospho‐PKD1 (serine 916) and β‐actin. (B) Quantification of blots from panel A. (C) Representative immunoblots of protein extracts from mouse islets stimulated for 20 min with KCl (30 mmol/L) ±P2Y₁ antagonist, MRS2500 (1 μmol/L) at 1‐mmol/L glucose and analyzed by Western blot for phospho‐PKD1 (serine 916) and β‐actin. (D) Quantification of blots from panel C. Data are mean ± SEM for three independent experiments, *P < 0.05 compared to the control condition.