Figure 2.

Potentiation of insulin secretion via activation of P2Y1 is PKD1 dependent. (A) Insulin secretion was measured from C57Bl/6 mouse islets over a 1‐h period at 1 and 10 mmol/L glucose in the presence of ATP (10 µmol/L) or MRS2365 (100 nmol/L). (B) There was no significant difference in glucose‐stimulated insulin secretion between control MIP‐Cre‐ERT mice (black) and mice lacking β cell PKD1 (βPKD1KO; red), but the βPKD1KO failed to respond to the P2Y₁ agonist, MRS2365 (100 nmol/L; open bars). (C) Insulin content assessed after the static incubations in MIP‐Cre‐ERT and βPKD1KO mouse islets was not different. Data are mean ± SEM for 3–5 independent experiments, *P < 0.05 and **P < 0.01 compared to the control condition or as indicated.