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. 2019 Sep 24;6(10):482–490. doi: 10.15698/mic2019.10.694

Figure 2. FIGURE 2: The stress-responsive transcription activator genes MSN2/MSN4 are involved in replicative lifespan. (A and B).

Figure 2

The expression of PUT1 in Δmsn2 Δmsn4 double deletion strain and MSN2-overexpressing strain at log and stationary phases compared with that in the wild-type strain (WT; BY4741) at log phase. The values are the means and standard deviation of results from three independent experiments. *, p < 0.05; **, p < 0.01, versus WT calculated with the Student's t-test. (C) Intracellular proline levels in the indicated strains compared with WT at stationary phase. The values are the means and standard deviation of results from three independent experiments. *, p < 0.05; **, p < 0.01, versus WT calculated with the Dunnett's test. (D) Replicative lifespan of the indicated strains. The data for WT was the same as Figure 1A and 1D. The results of other mutants, which were analyzed independently, and WT were combined.