TABLE 1.

Replicative lifespan of yeast mutants involved in proline metabolism.

Allele	Replicative lifespan
	Average	±	SD	Percentage change (%)	p valueb	Maximum	n
BY4741
WT	24.9	±	8.5	-	-	47	96
Δput1	30.5	±	7.9	22.5	4.2×10−6	47	96
PRO1-I150T	33.3	±	7.5	33.7	4.9×10−11	50	96
Δput1 PRO1-I150T	31.5	±	8.2	26.5	1.0×10−7	50	96
Δcar2	17.6	±	6.4	−29.3	1.1×10−8	30	96
Δpro1	17.3	±	4.9	−30.5	5.1×10−11	28	96
Δpro2	17.1	±	5.3	−31.3	5.9×10−11	29	96
MSN2(OE)a	16.3	±	4.9	−34.5	2.7×10−13	28	96
MSN2(OE)a Δput1	21.1	±	6.7	−15.3	3.2×10−3	38	96
Δmsn2 Δmsn4	23.8	±	8.8	−4.4	5.6×10−1	43	48
CAY29b
WT	22.6	±	7.3	-	-	43	48
Δput4 Δgap1 Δagp1 Δgnp1	23.0	±	8.3	1.77	6.3×10−1	41	48

^aMSN2(OE) represents overexpression of the MSN2 gene driven by the TDH3 promoter.

^bThe p value was calculated using a Wilcoxon rank-sum test relative to the wild-type strain (WT).