Figure 3.

Electrophysiological profile of MilIA [M9G, N10K]. The electrophysiological profile showed the ACh-evoked current mediated by α1β1γδ, α4β2, α7, α9α10, α4β4, and α1β1δε nAChRs. The nAChRs were gated by a variable time duration pulse of ACh at 2 mL/min. (red) (200 µM for α₁β₁γδ, α4β2, α4β4, α1β1δε; 100 µM for α7; 500 µM for α9α10) for different nAChR subtypes. The first and the second peak amplitude represented the absence and presence of 1 µM of MilIA [M9G, N10K], respectively. The conopeptide was applied for 60 s at 2 mL/min. (blue), immediately followed by a variable time duration pulse of ACh (red).