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Factors and levels used for the experimental design.
| Variables | Levels Used | Optimum Conditions | ||||
|---|---|---|---|---|---|---|
| Temperature (°C) | 60 | 70 | 80 | 100 | 70 | |
| Time (hr) | 1 | 2 | 3 | 2 | ||
| Concentration (% H2SO4 in methanol) | 3 | 5 | 7 | 9 | 5 | |
| Acid and toluene volume (mL) | 2 | 3 | 4 | 5 | 6 | 4 (each) |
| Sample weight (mg) | 100 | 200 | 300 | 400 | 500 | 300 |
| Moisture (%) | 0 | 2.5 | 5 | 7.5 | 0–7.5% | |
