Ex vivo expanded CD34+ cells maintain their colony-forming capabilities. Purified PB-CD34+ non-expanded cells, or cells expanded ex vivo in the presence or absence of NAC or Ech A for 4 days were evaluated for pluripotency using hematopoietic colony-forming assays in semi-solid methylcellulose media. (A) Colony-forming unit/burst-forming unit-erythroid (CFU/BFU-E), CFU-granulocyte/macrophage (CFU-GM), and CFU-granulocyte/erythroid/macrophage/megakaryocytes (CFU-GEMM) per 500 CD34+ cells plated were enumerated after 14 days of culture. (B) Representative images of CFU/BFU-E, CFU-GM, and CFU-GEMM for each experimental condition. Magnification: ×200.