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. 2019 Sep 3;7(9):313. doi: 10.3390/microorganisms7090313

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) LC-HRMS total ion chromatogram of the fraction containing piscibactin-Ga(III) complex eluted with H₂O and CH₃CN (1:1) from an OASIS^® HLB cartridge; (b) extracted ion chromatogram from m/z 519.7–520.4; (c) high resolution mass spectrum corresponding to the peak with t_R = 12.06 min; (d) isotopic ion abundance from m/z 516.0–523.5, including M+1/M (¹³C₁/¹²C), calculated with Thermo Xcalibur software 3.0. LTQ-Orbitrap was operating at a resolving power of 30,000 (m/Δm) with a detection window setting around the compound of interest (between 350 and 600 dalton).