Table 1.
Primers used in this work.
| Primers | Sequence (5′-3′) * | Amplified Fragment (bp) |
|---|---|---|
| Amplification of potential promoters | ||
| P1 | ||
| Promoter 1_F | GCGTCTAGACACTTTGCCACCCACCATTA | 879 |
| Promoter 1_R | GCGGGATCCACGAATCGTCGTGTTGGCAT | |
| P2 | ||
| Promoter 2_F | GCGTCTAGACCGCTTAGAGAAACCAACGT | 1165 |
| Promoter 2_R | GCGGGATCCACGTTTCGGTAAGCGTATGG | |
| Transcriptional regulation of irp gene cluster | ||
| RT | TTTGGAGATGAGTGCGACAC | |
| PCR1 | ||
| ARC1ordalii_F | GATATGCGCTTTGACTGCCA | 196 |
| ARC1ordalii_R | CTGTGAGACGGCATACAAGC | |
| PCR2 | ||
| FrpA_ordalii_F | CGGTGGTAATGCTCAAGGTG | 204 |
| FrpA_ordalii_R | TGGCTCGGTAGGTGTTCAAT | |
| PCR3 | ||
| Irp2_ordalii_F | AGCAGGCAACAAAGAGTGAG | 413 |
| Irp1_ordalii_R | GGGCGAATAACCAAACAAGC | |
* Recognition sequences for restriction enzymes are underlined.