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5-HT3 receptor antagonists function as novel autophagy inducers in lung cancer cells. A549 cells were treated with or without ondansetron (40 μg/mL), palonosetron (8 μg/mL), or ramosetron (4 μg/mL) for 24 h and then added to 50 nM bafilomycin A1 (Baf-1) 2 h before observation. The level of LC3B was determined by Western blotting. GAPDH served as a loading control; * p < 0.05 vs. untreated cells, # p < 0.05 vs. 5-HT3RA-treated cells.
