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Stem-loop RT-PCR confirmation of eight A. c. cerana miRNAs. Lane M: DNA marker; Lane N: Negative control (sterile water was used as PCR template); snRNA U6 was used as positive control. The upper panel presents the AGE of PCR amplification products of miR-1943-x, miR-3793-x, miR-252-y, and novel-m0014-3p; the lower panel displays the AGE of PCR amplification products of miR-3963-x, miR-8516-x, miR-149-y, and miR-7311-y. The complete gel was presented in Figure S3.
