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. 2019 Aug 21;10(9):258. doi: 10.3390/insects10090258

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Stem-loop RT-PCR and RT-qPCR validation of A. c. cerana DEmiRNAs. (A–C) Stem-loop RT-PCR validation of miR-6547-x, miR-7311-x, and novel-m0008-3p; (G–I) Stem-loop RT-PCR validation of miR-2779-y, miR-3726-x, and miR-1788-y; (M,N) Stem-loop RT-PCR validation of miR-3319-y and miR-1672-x; (D–F) RT-qPCR validation of miR-6547-x, miR-7311-x, and novel-m0008-3p; (J–L) RT-qPCR validation of miR-2779-y, miR-3726-x, and miR-1788-y; (O,P) RT-qPCR validation of miR-3319-y and miR-1672-x; snRNA U6 was used as a positive control in Stem-loop RT-PCR and reference gene in RT-qPCR. The experiment was performed three times using three independent biological samples. The left two charts showed the result of RT-qPCR of selected DEmiRNA (in light gray), while the right two charts showed the differential expression (log₂(Fold change)) of the same selected DEmiRNA from sRNA-seq data (in dark gray). RT-qPCR data are represented as the mean ± SD.

Stem-loop RT-PCR and RT-qPCR validation of A. c. cerana DEmiRNAs. (A–C) Stem-loop RT-PCR validation of miR-6547-x, miR-7311-x, and novel-m0008-3p; (G–I) Stem-loop RT-PCR validation of miR-2779-y, miR-3726-x, and miR-1788-y; (M,N) Stem-loop RT-PCR validation of miR-3319-y and miR-1672-x; (D–F) RT-qPCR validation of miR-6547-x, miR-7311-x, and novel-m0008-3p; (J–L) RT-qPCR validation of miR-2779-y, miR-3726-x, and miR-1788-y; (O,P) RT-qPCR validation of miR-3319-y and miR-1672-x; snRNA U6 was used as a positive control in Stem-loop RT-PCR and reference gene in RT-qPCR. The experiment was performed three times using three independent biological samples. The left two charts showed the result of RT-qPCR of selected DEmiRNA (in light gray), while the right two charts showed the differential expression (log₂(Fold change)) of the same selected DEmiRNA from sRNA-seq data (in dark gray). RT-qPCR data are represented as the mean ± SD.