Figure 5.

Bioprinter testing with alginate beads. Soft alginate beads were used as a spheroid surrogate as they could easily be punctured and placed on the bioprinter needles. (A) The beads were synthesized at an average diameter of 851 ± 15 µm, which was similar to the spheroid diameter. (B–D) Picture (B) showing beads aspirated onto the end of a 4 × 4 arrangement print head and (C) printed onto the needles in a single layer. (D) Two layers were also tested to ensure that multiple layers could be printed. Scale bars: (A) 500 µm; (B) 2 mm; (C) and (D) 5 mm.