Table 2.

Published PCR assays used in this survey. The table includes the targeted gene, primer sequence and positive controls.

Pathogen	Target Gene	Primer Sequences 5’-3’	Positive Control	Reference
Anaplasma/Ehrlichia	groESL	gro607F GAAGATGC(A/T)GT(A/T)GG(A/T)TGTAC(G/T)GC gro677F ATTACTCAGAGTGCTTCTCA(A/G)TG gro1294R AG(A/C)GCTTC(A/T)CCTTC(A/T)AC(A/G)TC(C/T)TC gro1121R TGCATACC(A/G)TCAGT(C/T)TTTTCAAC	E. chaffensis	[40]
Borrelia	Flagellin b	FlaLL ACATATTCAGATGCAAGACAGA FlaRL GCAATCATAGCCATTGCAGATTGT FlaLS AACAGCTGAAGAGCTTGGAATG FlaRS CTTTGATCACTTATCATTCTAATAGC	B. lonestari	[41]
Rickettsia	ompA	Rr 190.70p ATGGCGAATATTTCTCCAAAA 190.701 GTTCCGTTAATGGCAGCATCT	R.sp endosymbiont	[42]