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. 2019 Aug 21;7(9):275. doi: 10.3390/microorganisms7090275

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biochemical analyses of Azospirillum brasilense 1224^T harboring the plasmid pHRGFPGUS converting morphologically into the c-form during incubation in the nitrogen-restricted MMAB medium. The cells were grown at 30 °C for three and seven days in the MMAB () or nitrogen-restricted MMAB medium (■), and the levels of poly-β-hydroxybutyrate (PHB), indole-3-acetic acid (IAA), and acetylene reduction activity (ARA) in the cells were analyzed as described in Materials and Methods. Values are expressed as the means ± standard deviation (SD) of three replicates. Means in the same parameter (PHB, IAA, ARA) with different letters (a, b, c) above each bar are significantly different from each other at p < 0.05 by Tukey’s HSD test. N.D., not detected.

Inline graphic — Biochemical analyses of Azospirillum brasilense 1224^T harboring the plasmid pHRGFPGUS converting morphologically into the c-form during incubation in the nitrogen-restricted MMAB medium. The cells were grown at 30 °C for three and seven days in the MMAB () or nitrogen-restricted MMAB medium (■), and the levels of poly-β-hydroxybutyrate (PHB), indole-3-acetic acid (IAA), and acetylene reduction activity (ARA) in the cells were analyzed as described in Materials and Methods. Values are expressed as the means ± standard deviation (SD) of three replicates. Means in the same parameter (PHB, IAA, ARA) with different letters (a, b, c) above each bar are significantly different from each other at p < 0.05 by Tukey’s HSD test. N.D., not detected.