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pSUPER-non/pSUPER-Becl 转染SW620细胞后Beclin1和GAPDH的RT-PCR扩增结果
RT-PCR amplification of Beclin1 and GAPDH in SW620 cells transfected by pSUPER-non and pSUPER-Becl
| Beclin1(Ct) | GAPDH(Ct) | △Ct | △△Ct | 2-△△CT | Mean | mRNA inhibition ratio(%) | |
| Compared to the control group, the Beclin1 mRNA inhibition of pSUPER-non group is 6.19%, #P>0.05; Compared to the control group, the Beclin1 mRNA inhibition of pSUPER-Becl group is 81.05%, *P<0.01. | |||||||
| Control | 24.80 | 17.25 | 7.56 | 0 | 1.00 | 1.08 | |
| 25.49 | 18.16 | 7.33 | -0.22 | 1.17 | |||
| pSUPER-non | 25.47 | 18.06 | 7.40 | -0.15 | 1.11 | 1.02 | 6.19# |
| 26.08 | 18.41 | 7.68 | 0.12 | 0.92 | |||
| pSUPER-Becl | 26.28 | 16.49 | 9.79 | 2.24 | 0.21 | 0.21 | 81.05* |
| 26.13 | 16.24 | 9.89 | 2.34 | 0.20 | |||