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. 2017 Mar 20;37(3):373–377. [Article in Chinese] doi: 10.3969/j.issn.1673-4254.2017.03.16

1.

pSUPER-non/pSUPER-Becl 转染SW620细胞后Beclin1和GAPDH的RT-PCR扩增结果

RT-PCR amplification of Beclin1 and GAPDH in SW620 cells transfected by pSUPER-non and pSUPER-Becl

Beclin1(Ct) GAPDH(Ct) △Ct △△Ct 2-△△CT Mean mRNA inhibition ratio(%)
Compared to the control group, the Beclin1 mRNA inhibition of pSUPER-non group is 6.19%, #P>0.05; Compared to the control group, the Beclin1 mRNA inhibition of pSUPER-Becl group is 81.05%, *P<0.01.
Control 24.80 17.25 7.56 0 1.00 1.08
25.49 18.16 7.33 -0.22 1.17
pSUPER-non 25.47 18.06 7.40 -0.15 1.11 1.02 6.19#
26.08 18.41 7.68 0.12 0.92
pSUPER-Becl 26.28 16.49 9.79 2.24 0.21 0.21 81.05*
26.13 16.24 9.89 2.34 0.20