Figure 1.

Inhibitory effects of oligo-fucodian (OF) on cell viability and colony formation of human malignant glioma cells. (A) Two malignant glioma (MG) cell lines (GBM8401 and U87MG) and immortalized astrocyte SVGp12 cells were treated with various concentrations of OF for 72 h. The cell proliferation was measured by sulforhodamine (SRB) assay. Values are expressed as the mean ± standard error of triplicate wells. (B) Effects of OF on the clonogenicity of GBM8401, and (C) U87MG cells. Each experiment was performed in triplicate, and the representative examples are shown (column, mean, bar, standard error; ** p < 0.01; *** p < 0.001). The IC₅₀ indicates the 50% inhibitory concentration (μg/mL) of OF in the 12-day clonogenicity assay of GBM8401 and U87MG cells, respectively. Data are expressed as mean ± standard error.