Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 23;8(9):1289. doi: 10.3390/jcm8091289

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Ibudilast inhibits LPS-induced osteoclast differentiation in vitro. (A) Representative image illustrating the inhibitory effect of ibudilast on the differentiation of cathepsin K-positive osteoclasts. RAW264.7 cells were cultured for 5 days with LPS (100 ng/mL) in the presence of ibudilast (10 μM) and subsequently stained for cathepsin K. (B) Quantitative data analysis confirmed the inhibitory effect of ibudilast against the formation of cathepsin K-positive multinucleated (≥3 nuclei) cells in vitro. (C) Representative image illustrating the inhibitory effect of ibudilast on the differentiation of TRAP-positive osteoclasts. (D) Quantitative data analysis confirmed the inhibitory effect of ibudilast against the formation of TRAP-positive multinucleated (≥3 nuclei) cells in vitro. Data are presented as means ± standard errors of the mean. Analyses were conducted with unpaired Student’s t-tests. * p < 0.05, ** p < 0.01. LPS, lipopolysaccharide; TRAP, tartrate-resistant acid phosphatase.