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. 2019 Sep 1;9(9):1905–1921.

Figure 4.

Figure 4

SLFN12 was required for the ANA-induced growth inhibition of cancer cells. A. Quantitative Real-Time PCR analyses of SLFN12 mRNA expression in 13 cancer cell lines. The mRNA levels were normalized to the GAPDH mRNA expression. B. Effects of SLFN12 siRNA on the mRNA expression of SLFN12. The H4 cells were transiently transfected with the SLFN12-specific siRNAs targeting three different sequences or non-specific scrambled siRNA. SLFN12 mRNA expression was measured by real-time PCR 48 h after siRNA transfection. C. RTCA analyses of the effects of knocking down SLFN12 expression on the ANA-induced growth inhibition. The siRNA-transfected H4 cells were cultured with ANA (100 nM) or DMSO and the growth of the cells were recorded by RTCA. D. The H4 cells were transiently transfected with the SLFN12-specific siRNA, PDE3A-specific siRNA, or a combination of the two with or without ANA treatments and the cell growths were recorded by RTCA.