Figure 7.

ANA inhibited the growth of tumor xenograft in vivo. Human tumor xenografts of H4 cells were established by subcutaneously inoculating the cells into female BALB/c-nude mice. The mice were orally administered with ANA (10 mg/kg or 30 mg/kg) or vehicle daily for 15 days when the tumor volumes reached 100 mm³. A. Photograph of the tumor-burdened mice after 15 days of ANA treatment. B. Body weight curves of the tumor-burdened mice. C. The tumor growth curves of the tumor-burdened mice. The tumors were measured and analyzed by two-way ANOVA. Data were presented as means ± s.d. (n = 6), ** P < 0.01, **** P < 0.0001. D. Photograph of the tumors from the control and ANA-treated mice. Mice were sacrificed after 15 days ANA treatment and the tumors were dissected and photographed. E. The weights of the individual tumors and their means.