Skip to main content
. 2019 Sep 1;9(9):1938–1956.

Figure 1.

Figure 1

Effects of MYCN on the chemoresistance of SCLC in vitro. A. cDNA expression profile showed that MYCN is differentially expressed between H69AR cells and H69 cells. B. RT-qPCR and Western blot analysis of MYCN expression in H69 and H69AR cells. C. Western blot analysis of MYCN expression in eight SCLC cell lines (H69, H69AR, H446, H146, H526, H345, H209, and H82). D. The cellular localization of MYCN was confirmed by immunofluorescence staining of H69AR cells. E, F. RT-qPCR and Western blot analyses of MYCN expression in H69AR and H526 cells transfected with siRNA targeting MYCN or NC siRNA and in H69 and H446 cells transfected with pcDNA3.1-MYCN or NC plasmids. G-J. CCK-8 assays showed that MYCN knockdown decreased the IC50 values of the chemotherapeutic agents (ADM, CDDP, and VP-16) in H69AR and H526 cells, whereas MYCN overexpression increased the IC50 values of these compounds in H69 and H446 cells. Error bars indicate the mean ± SD from three independent experiments. *, P < 0.05; ***, P < 0.001.