Figure 3.

DNA vaccine pEKL6 reduces tumor growth and prolongs survival time in WT mice. (A and B) WT mice (n = 5 per group) were I.M. immunized twice by electroporation with the indicated DNA vaccines. EL4-L6 cells (2×10⁵) were injected subcutaneously at 7 days after final immunization. Tumor volume was detected 2-3 times per week (A), and the survival rate was observed (B). (C and D) Mice were injected with B16-L6 cells (2×10⁴) subcutaneously 7 days after the final immunization. The tumor volume was measured (C), and the survival rate was observed (D). (E) The surviving mice in the pEKL6 group from (D) were subcutaneously transplanted tumors again on day 90.