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. 2019 Sep 1;9(9):1970–1981.

Figure 2.

Figure 2

UCHL3 deubiquitinates FOXM1. (A) Cells were transfected with HA- FOXM1, FLAG-UCHL3, and Myc-Ub as indicated. The polyubiquitylated FOXM1 protein was detected by anti-Myc antibody. (B) Cells stably expressing control or UCHL3 shRNAs were subjected to deubiquitination assay and the polyubiquitylated FOXM1 protein was detected by the anti-Ub antibody. (C) Cells transfected with FLAG-FOXM1 were treated with or without TCID. The polyubiquitylated FOXM1 protein was examined as in (B). (D) Deubiquitination of FOXM1 in vitro by UCHL3. Ubiquitinated FOXM1 was incubated with purified UCHL3 or UCHL3CA in vitro and then immunoblotted with the indicated antibodies.