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. 2019 Sep 2;9(9):1246. doi: 10.3390/nano9091246

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hyaluronan functionalization increases the nanoparticles interaction, uptake in HT-29 cells. Flow cytometry histogram shows alone HT-29 cells, HT-29 cells treated with Cur-HA-NPs signal after 3 h incubation (A). Representative ImageStream flow cytometry images show HA functionalized particles has effective uptake in HA-29 cells (B). Representative slide scanner images of uncoated Cur NPs, Cur solution and Cur-HA NPs after 3 h incubation, HA functionalized NPs showing more cellular interactions and uptake compared to uncoated NPs; nuclei (blue) stained with DAPI, cytoskeleton (red) stained with rhodamine-phalloidin, curcumin (green) (C).