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. 2019 Sep 16;17(9):536. doi: 10.3390/md17090536

Figure 2.

Figure 2

Individual cytotoxicity of renieramycin M (RM) and doxorubicin (DOX) on MCF-7 breast cancer cells. (A) Concentration-dependent cytotoxicity of RM and DOX from MTT cytotoxicity assay at 72 h post-treatment. Data points are mean ± SEM of three independent trials performed in quadruplicates. *** p < 0.0001 (one-way analysis of variance—ANOVA/Dunnett’s multiple comparison test). (B) Slopes of the concentration-response curves. m = “shape” or the slope of the curve; r = conformity of the data to the mass-action law. These were automatically generated using the CompuSyn software (Paramus, NJ, USA) [12]. (C) Time-dependent cytotoxicity of RM and DOX using xCELLigence® software (RTCA; ACEA, Biosciences Inc., San Diego, CA, USA) during a seven-day exposure. Each data point represents mean IC50 ± SEM (n = 3) calculated during the indicated time-points of a representative experiment. Solid black line indicates the time of treatment; Δt24 and Δt48 indicate time points after 24 and 48 h of exposure, respectively.