Figure 6.
Transcriptome profile of MCF-7 treated with RM and DOX, singly and in combination. RNAs from MCF-7 cells treated with RM (6.25 nM) and DOX (313 nM) and its combination for 60 h were hybridized in an Affymetrix PrimeView Human Gene Expression Array. The single drug treatments and negative control (0.1% DMSO + sdH2O) were performed in duplicates while the combination treatment in triplicates. (A) Volcano plots showing the differentially expressed genes (DEGs). A threshold of two-fold upregulation (red) or downregulation (blue) relative to the negative control (Neg), with false discovery rate (FDR) p < 0.05 were used to filter the significant DEGs. (B) Principal component analysis and (C) hierarchical clustering of DEGs showing signature gene expression profiles of RM, DOX, and RM + DOX and the top 15 significantly enhanced or repressed genes upon combination treatment. (D) Venn diagram analysis showing the number of DEGs common or specific between treatments.