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(A) Dead cells were scored as necrotic (Annexin V-negative/7-AAD-positive, upper left quadrants, Q1), late apoptotic (Annexin V-positive/7-AAD-positive, upper right quadrants, Q2), early apoptotic (Annexin V-positive/7-AAD-negative, lower right quadrants, Q3), and a gating on the normal cells that are considered viable, are PE Annexin V and 7-AAD negative (lower left quadrants, Q4). B. Apoptosis percentage was indicated as a bar chart (mean ± SEM, no statistical difference was noted).
