Figure 5.

WT B6 mice were made neutropenic (PMN^−/−) by intraperitoneal injection of Anti-Ly6G-1A8 antibody. Immunocompetent B6 mice (without PMN depletion, PMN^+/+) were used as control. Then, 24 h following neutropenia eyes (n = 10 each time point) were infected with A. fumigatus spores (15,000 CFU/eye) by intravitreal injections. (A) Slit-lamp examination was performed at indicated time points and photomicrographs were taken from representative eyes. (B) For histological analysis, eyes were enucleated at indicated DPI and subjected to H&E staining. (C) Fungal burden was estimated from eye lysates via serial dilution plating. (D) The eye lysate was subjected to ELISA for representative inflammatory cytokine e.g., IL-1β. ns, not significant; **, p < 0.005; ***, p < 0.0005; One-way ANOVA. R: retina, OD: optic disc, L: lens, C: cornea.