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. 2019 Jul 25;216(10):2331–2347. doi: 10.1084/jem.20190164

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© 2019 Bancroft et al.

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Figure 1. — Identification and characterization of anti-iglb12 idiotypes. (A) BLI analysis of the purified products of four hybridomas named IB1, IB2, IB3, and IB5 binding to different concentrations of iglb12. (B) The affinity (K_D) of the anti-idiotype for iglb12 was calculated based upon the on rate (K_ON) and off rate (K_OFF) determined by BLI. (C) The heat map displays the maximum shift (nm) measured by BLI when IB1, IB2, IB3, or IB5 were incubated with the listed antibodies and antibody groups. Detailed information on sequences of the non–V_H1-3 and V_H1-3⁺ control antibodies can be found in Table S1. Data are representative of two to three similar experiments.