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. 2019 Jul 25;216(10):2331–2347. doi: 10.1084/jem.20190164

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© 2019 Bancroft et al.

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Figure 8. — In vitro response of transgenic B cells expressing the iglb12 heavy chain. (A and B) Representative flow cytometric analysis (A) and quantitation of the percentage of CTV^DILUTED B cells (B) from individual iglb12 heavy chain transgenic (Tg) or WT control mice were labeled with CTV following in vitro culture in the presence of 2, 5, 10, or 25 µg/ml polyclonal goat anti-mouse Ig or media alone for 72 h. The percentages of B cells in the gates are shown in A. (B) Mean percentage ± SD of CTV^DILUTED cells found in samples from seven individual mice from three independent experiments. The P values (*, P < 0.05; **, P < 0.01; ***, P < 0.001) were determined using an unpaired two-tailed Student’s t test.