Table 1. Percentages of lung tryptase activity due to α/β-tryptase after purification of tryptase by B2-agarose immunoaffinity chromatography (P0).
| Genotype | Tryptase activity in P2 (%)a | Tryptase activity based on stability to B12 inhibition (%)b | n |
|---|---|---|---|
| αβ:ββ | 15 ± 2 | 15 ± 1 | 3 |
| αβ:αβ | 30 ± 7 | 46 ± 5 | 3 |
| ββ:ααβc | 27 | 27 | 1 |
| ββ:αααβc | 37 | 43 | 1 |
Values are presented as mean ± SD. Data represent two replicate experiments for each of the three different individuals with an αβ:ββ or αβ:αβ tryptase genotype in column 2, triplicate values for each of the three different subjects with an αβ:ββ or αβ:αβ tryptase genotype in column 3, two replicates from the one subject with a ββ:ααβ or a ββ:αααβ tryptase genotype in column 2, and three replicates from the one subject with a ββ:ααβ or a ββ:αααβ tryptase genotype in column 3.
a
100 × P2/(P1 + P2).
b
Percentage activity after 20 min compared to β-tryptase (P0, ββ:ββ genotype) and α/β-tryptase (P2, ββ:αααβ genotype).
c
Hereditary α-tryptasemia genotype.