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. 2019 Jul 26;216(10):2253–2264. doi: 10.1084/jem.20190896

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© 2019 Gaebler et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — Predicted detection of HIV-1. (A) Horizontal bars represent the predicted detection of 578 intact clade B proviral sequences (Los Alamos HIV sequence database) by qPCR primer/probe sets that target PS (green), gag (blue), pol (yellow), and env (red) regions. Signal prediction for each individual proviral sequence is represented by the presence of the color of the respective primer/probe set. Sequences containing polymorphisms that prevent signal detection are represented by the absence of color. The percentage indicates the fraction of detected sequences for individual primer/probe sets or combinations of two primer/probe sets (brackets). (B) Horizontal bars represent the predicted detection of 401 intact and 977 defective NFL genomes from nine individuals (Lu et al., 2018; Mendoza et al., 2018). The same primer/probe sets and color scheme are used as described above. The group of defective sequences includes NFL genomes that carry small insertions, deletions, and defects in the packaging site and/or MSD. The length of the scale bar represents 10 proviral sequences.