Table 1.

Variation in the ratios of broken to unbroken bands in each F2 and backcross (BC to H. m. meriana) mapping family

Family	Family size	Relaxed threshold			Stringent threshold
		Broken	Unbroken	P-val.	Broken	Unbroken	P-val.
B5: F2	35	5	30	0.143	4	31	0.064
B8: BC	112	34	78	<0.001	34	78	<0.001
B10: F2	82	17	65	0.372	12	70	0.039
B11: F2	50	4	46	0.006	4	46	0.006
B12: F2	66	14	52	0.47	13	53	0.32
B13: F2	57	11	46	0.32	8	49	0.055
B14: BC	154	75	79	0.747	74	80	0.628

Bands are scored using the relaxed threshold of ≥2 for presence of a broken band and using the stringent threshold for presence of a broken band (scoring methodology detailed in methods). In bold are chi-square P-values for the two scoring methods showing significant deviation from the expected ratio (3:1 for F2 cross; 1:1 for backcross) for a phenotype (unbroken:broken) controlled by a single Mendelian locus. B10 and B14 were used for the QTL analysis