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. 2019 Sep 12;7(9):350. doi: 10.3390/microorganisms7090350

Figure 1.

Figure 1

(a) Zika virus (ZIKV) infects A549 cells and elicits expansion of Vδ2 T-cells. A549 cells were infected with ZIKV MR766 strain and the frequency of ZIKV+ cells was analyzed by flow cytometry using the PANFLAVI antibody after 24, 48, and 72 h. Flow cytometry panels of one representative experiment out of 15 are shown. PANFLAVI+ gate was set to include ≤0.2% of events when using primary or secondary antibody isotype controls or uninfected cells (mock). (b) Expansion index of Vδ2 T-cells in co-culture with ZIKV-infected cells was shown. A549 cells were not infected (mock) or infected with ZIKV MR766 and cultured with peripheral blood mononuclear cells (PBMC) of healthy donors (HD). IL-2 or IL-2 + PhAg were added in parallel cultures as controls. Vδ2 T-cells frequency was calculated at baseline (T0) and after 7 days (T7). The expansion index was calculated by dividing Vδ2 T-cells frequency at 7 days in respect to T0. Results from 13 independent experiments are shown. Data were represented as median + interquartile range. * p < 0.05; ** p < 0.01.