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. 2019 Sep 12;7(9):350. doi: 10.3390/microorganisms7090350

Figure 2.

Figure 2

ZIKV sensitized A549 cells to Vδ2 T-cell-mediated killing. (a–c) Expanded Vδ2 T-cells were cultured overnight with mock (white bars) or ZIKV-infected A549 cells (grey bars) cells, at 1:1 and 5:1 ratios. (a,b) After 18 h, apoptotic/necrotic A549 cells were analyzed by measuring AnnexinV and PI expression by flow cytometry (n = 15). (c) The frequency of Vδ2 + /CD107a+ cells was measured after the culture with mock/ZIKV-infected cells (n = 7). (d) Perforin quantification in the supernatants of the same experiments was shown. In all experiments, background (mock/ZIKV+ apoptotic/necrotic cells without expanded Vδ2 T-cells) was subtracted to all conditions. Data were represented as median + interquartile range. * p <0.05; ** p < 0.01.