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. 2019 Sep 12;7(9):350. doi: 10.3390/microorganisms7090350

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

ZIKV up-modulated NKG2DLs in A549 cells. (a–c) Expression of NKG2DLs was analyzed in A549 cells infected or not for 48 h with ZIKV (MOI 1). (a) Relative NKG2DL mRNA levels in ZIKV-infected cells compared with control mock cells were measured by Real-time qPCR (n-5). (b,c) The frequency of MICA/B +, ULBP-1 +, and ULBP-2+ was measured on mock/ZIKV-infected cells. (b) Histograms show MICA/B, ULBP-1, and ULBP-2 expression in a representative experiment. (c) Median ± Range of NKG2DL+ cells were calculated in 8 independent experiments. Data were represented as median + interquartile range. * p < 0.05; ** p < 0.01.