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. 2019 Sep 12;7(9):350. doi: 10.3390/microorganisms7090350

Figure 5.

Figure 5

ZIKV sensitizes A549 cells to NKG2D-mediated killing by Vδ2 T-cells. (a) Flow cytometric panels showing the expression of NKG2D receptor on Vδ2 T-cells ex vivo and after 12 days of PhAg + IL-2 stimulation (Vδ2 T line) in a representative experiment. (b) The frequency of NKG2D+ cells among Vδ2 T-cells was compared in 9 experiments at T0 (white bars) and T12 days (squares bars). (c) Flow cytometry panels showing the expression of AnnexinV on mock/ZIKV-infected A549 cells after co-culture at E:T ratio of 1:1 with Vδ2 T-cells pre-treated or without blocking a-NKG2D antibody. (d) Comparative analysis of 9 independent experiments as the one depicted in panel C is shown. (e) Flow cytometry panels showing the expression of PI on mock/ZIKV-infected A549 cells after co-culture at E:T ratio of 1:1 with Vδ2 T-cells pre-treated or without blocking a-NKG2D antibody. (f) Comparative analysis of 8 independent experiments as the one depicted in panel E is shown. In all experiments, background (mock/ZIKV+ apoptotic/necrotic cells without expanded Vδ2 T-cells) was subtracted to all conditions. Data were represented as median + interquartile range. * p < 0.05; ** p < 0.01; *** p < 0.001.