Figure 2.

Uptake (A) and transport (B) studies using iPSC-MBECs demonstrated that biphalin uptake in the endothelial cells (ECs) and transport across the blood-brain barrier (BBB) decreased in the presence of OATP1 inhibitors. (A) For uptake studies, the cells were pre-incubated with estrone-3-sulfate (E3S) for 10 min. before incubating with mixture of biphalin (10 µM) and inhibitor (E3S, 10 µM) for 20 min. (B) For transport studies, monolayer cells were pre-treated 30 min. with OATP1 inhibitor. A mixture of biphalin and inhibitor was added to the apical side and samples were collected from basolateral side (A to B transport). FITC-dextran 4kD was used to correct the paracellular permeability. Presented permeability coefficient (PC) is the net difference of PC of biphalin and FITC-dextran. Data represented as Mean ± SD (n = 4). * p < 0.05.