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Cytotoxic efficiency of the Mel-alone and 18:1βCDg-Mel complexes (at 2:1 molar ratio) in 3D spheroid A375 melanoma cells. A375 cells were seeded at 1 × 104 cells/well in Corning® spheroid 96-well microplates and treated twice (24 h and 36 h after seeding) with Mel-alone and 18:1βCDg-Mel complexes with final Mel concentrations of 30 µM and 80 µM. Cell toxicity was reported as lactate dehydrogenase (LDH) activity. N = 3 ± SD * indicates significance (P < 0.05).
