Table 1.
Lipid composition of the different liposomal formulations used throughout the work as well as the concentration and hydration and extrusion temperature used for their preparation. CHOL: cholesterol; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPG: 1,2-dimyristoyl-sn-glycero-3-phosphorylglycerol, DODAB: dioctadecyldimethylammonium bromide, DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine, DPPC: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DSPC: 1,2-distearoyl-sn-glycero-3-phosphocholinephosphatidylcholine, TDF: tenofovir disoproxil fumarate.
| Type of Studies | Lipid Composition | Concentration (mM) | Temperature Used in Hydration and Extrusion Process (°C) |
|---|---|---|---|
| Mucoadhesion studies | Zwitterionic liposomes:DMPC | 0.4 | 40 |
| Anionic liposomes:DMPG | 40 | ||
| Cationic liposomes:DODAB | 60 | ||
| Encapsulation of TDF (40 μM) | DOPC | ||
| DOPC:CHOL (7:3) | 4 | Room temperature | |
| DOPC:CHOL (6:4) | |||
| DMPC | |||
| DMPC:CHOL (7:3) | 4 | 40 | |
| DMPC:CHOL (6:4) | |||
| DPPC | |||
| DPPC:CHOL (7:3) | 4 | 55 | |
| DPPC:CHOL (6:4) | |||
| DSPC | |||
| DSPC:CHOL (7:3) | 4 | 65 | |
| DSPC:CHOL (6:4) |