Figure 3.

Impact of lipid phase transition temperature on formation and delivery efficiency of SP-B containing proteolipid-coated nanogels. (a) Evaluation of eGFP silencing in H1299_eGFP cells by uncoated or (proteo)lipid-coated siNGs with different lipid mixtures, supplemented with 0.4 wt% SP-B. All experiments were performed with a fixed NG concentration (30 µg/mL) and siRNA concentration (50 nM). The SP-B effect is strongly influenced by the type of lipid with which it is combined, highlighting the importance of a fluid lipid membrane in the formulation of the core-shell nanocomposites. (b) Chemical structures and phase transition temperatures (Tc) of the different PC lipids tested. Statistical analysis was performed via an unpaired t-test. Data are represented as the mean ± SD (n = 2) and statistical significance is indicated (**** p < 0.0001, ns = not significant).