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. 2019 Aug 23;11(9):431. doi: 10.3390/pharmaceutics11090431

Figure 6.

Figure 6

Impact of dextran nanogel core structure on SP-B mediated siRNA delivery. (a) Gene silencing potential of (proteolipid-coated) siRNA-loaded NGs (siNGs) constructed with the hydrolysable dex-HEMA or the stable dex-MA. Both formulations were coated with a mixture of DOPC:PG (85:15 wt%) here abbreviated as LIP, with or without SP-B. Although the stable dex-MA shows a less pronounced eGFP knockdown, SP-B promotes siRNA delivery equal to the degradable dex-HEMA. Data are a summary of two independent experiments. Data are represented as the mean ± SD (n = 2) and statistical significance is indicated (** p < 0.01, *** p < 0.005, ns = not significant). (b) Gene silencing of (proteo)lipid-coated dex-HEMA NGs with an intact or degraded NG core. All experiments were performed with a fixed NG concentration (30 µg/mL) and siRNA concentration (5 nM). Data show one representative graph of two independent experiments; formulations with increased SP-B fraction showed the same trend (data not shown).