Figure 2.

Intracellular localization of pHPMA polymer conjugate labeled with Dyomix 630 (A), Cy5 (B), ATTO647 (C), and Cyanine 5.5 (D). The HeLa cells were incubated for 24 h with the labeled polymers. The amount of fluorescently labeled polymer conjugates added to the cell suspensions was normalized to the dye content (1 μg/mL). The fluorescently labeled polymers (red color) were excited at 647 nm, and the emitted light was detected using a 640–740 nm filter. Nuclei were visualized by labeling with Hoechst 33342 dye excited at 405 nm, and emitted light was detected using a 425–500 nm filter, scale bar 20 µm.