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AKT protein expression in MCF-7 cells. Cells were treated with media (untreated), carbonate apatite, free AKT siRNA, CA/AKT siRNA, CA/AKT/Pac, and CA/Pac. Ingredients include 3 mM of CaCl2, 1 nM siRNA, and 1 nM Pac. Cellular lysates were run in SDS-PAGE and transferred into PVDF membrane and incubated with primary antibodies raised in rabbit against AKT. HRP-conjugated goat anti-rabbit secondary antibody was used to detect the chemiluminescent signals.
