Fig. 6.

d-Limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR during fed-batch cultures on glucose. Fed-batch shake flask cultivation was carried out at 20 °C, 250 rpm. The fermentation condition of fed-batch fermentation in the fermenter was performed at 28 °C, pH 5.7 and with 20% dissolved oxygen. 50% glucose solution was continuously fed into the shake flask and fermenter (1 mL at every 24-h time-point and 120 mL over every 24-h cultivation period, respectively). All values presented are the mean of three biological replicates ± standard deviation