Fig. 5.

The association between differential expression and DNA methylation patterns in young animals is not random. a Distribution of the correlation coefficients generated by correlating log2 fold mRNA change with gene body methylation of 500 randomly sampled genes (N = 10,000). Arrow indicates the location of the correlation coefficient of gene body methylation and differentially expressed genes in males. Snippet showing the polynomial regression curves of randomly selected gene sets compared to that observed in males (black regression line). b Correlation between age-related differential gene expression and gene body methylation of Reactome pathways gene sets (only pathways with > 50 genes are included). Regression curve through all differentially expressed genes with aging and gene body methylation in males is shown in black. Distributions of the correlation coefficients generated by correlating log2 fold mRNA change with promoter (c) or gene body methylation (d) for each Reactome pathway gene set