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. 2019 Oct 7;18:342. doi: 10.1186/s12936-019-2980-y

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — CQ_GREEN uptake in CQS and CQR parasites. Trophozoite stage parasites were incubated with CQ_GREEN and fluorescence was recorded every 3 s. a Fluorescence was measured in the parasite cytosol, DV, infected RBC (iRBC) cytoplasm and uninfected RBC (uRBC). No accumulation of CQ_GREEN was observed for the uRBC and iRBC cytoplasm. Uptake rates were calculated for the parasite cytosol and DV during the saturation phase (black curve), which was followed by a linear uptake phase. b CQR strains showed a slower CQ_GREEN uptake rate compared to CQS strains in both the cytosol and the DV of the parasite. Addition of verapamil (VP) did not influence the CQ_GREEN uptake rate in CQR strains. Experiments were done in triplicate